Biotium CF™ Dye Technology

CF488A Dye

超越Alexa Fluor 488的优质绿色抗体标记荧光染料

        CF488A 是一种针对 488nm 氩离子激光器优化的绿色荧光染料。在通常的检测条件下,CF488A 的亮度至少和 Alexa Fluor 488 相当。但 CF488A 相对于Alexa Fluor 488 有一个明显的优势,用CF488A 制备的荧光偶联抗体的生物学特异性更好。Alexa Fluor 488 本身带有多个负电荷,将会显著的改变被标记蛋白的等电点,因此也就会改变用它标记的荧光偶联抗体的生物学特异性。与此相反,CF488A几乎不带电荷,用它制备的荧光偶联抗体可以保证检测的特异性并提高信噪比。CF488A 的另一个优点是它的峰值发射波长比 Alexa Fluor 488短10nm,比传统的FITC(FAM)染料短15nm。因此,在多波长检测的时候,CF488A 可以显著减少在红色通道的溢出效应,从而减小对红色荧光的干扰。

        表A-C 为 CF488A 产品目录,我们提供全套的 CF488A 荧光偶联二抗,抗体标记试剂盒,以及其他生物分子偶联 CF488A 的产品,包括:鬼笔环肽,膜联蛋白 V和a-银环蛇毒素等偶联产品。

        更多产品信息可以从Biotium公司网站上获取:www.biotium.com

技术资料:

优势:


 

 图1.CF488A(蓝色), Alexa Fluor 488(绿色)和FITC(红色)偶联羊抗鼠IgG 二抗在PBS溶液中的激发和发射光谱。

 

图2. 用胞外CD3抗体或同型对照抗体(BD Biosciences)分别标记Jurkat细胞,然后分别用偶联图中所示厂商荧光基团的羊抗鼠IgG二抗染色。插图显示的是用Beckman Coulter FC-500检测到的细胞计数的中位数荧光值。直方图显示的是非别用CF488A(绿色),AlexaFluor488(蓝色),DyLight488 Pierce(紫色)和DyLight488 KPL(红色)染色的CD3标记细胞,使用BD FACS Calibur 在FL1通道上检测。

CY2,alexa fluor 488,抗体染料,一抗,二抗,山羊抗大鼠IgG,山羊抗小鼠IgG,山羊抗鼠IgG,兔抗山羊IgG,兔抗大鼠IgG,兔抗鼠IgG,山羊抗鸡IgG,驴抗山羊IgG,驴抗鼠IgG,驴抗大鼠IgG,驴抗羊IgG,驴抗绵羊IgG,山羊抗兔IgG,羊抗人IgG,蛋白质,偶联抗体

图3. 用fibronectin抗体标记人的心肌冰冻切片,并分别使用CF488A,Cy 2 和Alexa Fluor 488偶联的羊抗兔IgG作为二抗染色。感谢来自德国Bad Nauheim (Hessen) Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut)的Sawa Kostin博士提供了上述图片。

订货信息:

编号 产品名称/规格

价格(rmb)

生产商
表一:CF488A 标记的二抗
20010
CF488A goat anti-mouse IgG (H+L), 2 mg/mL    0.5 mL
¥2250 Biotium Inc.
20011
CF488A F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL    0.25 mL
¥1800 Biotium Inc.
20012
CF488A goat anti-rabbit IgG(H+L), 2 mg/mL    0.5 mL
¥2250 Biotium Inc.
20013
CF488A F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL    0.25 mL
¥1800 Biotium Inc.
20014
CF488A donkey anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5 mL
¥2700 Biotium Inc.
20015
CF488A donkey anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5 mL
¥2700 Biotium Inc.
20016
CF488A donkey anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5 mL
¥2700 Biotium Inc.
20017
CF488A goat anti-guinea pig IgG(H+L), 2 mg/mL    0.5 mL
¥2250 Biotium Inc.
20018
CF488A goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20019
CF488A goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20020
CF488A goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20021
CF488A rabbit anti-goat IgG(H+L), 2 mg/mL    0.5mL
¥2250 Biotium Inc.
20022
CF488A goat anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20023
CF488A goat anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20024
CF488A donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20025
CF488A rabbit anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20026
CF488A rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20293
CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20302
CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL    0.5mL
¥3525 Biotium Inc.
20027
CF488A donkey anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20028
CF488A goat anti-swine IgG(H+L), 2 mg/mL    0.5mL
¥2250 Biotium Inc.
20071
CF488A Rabbit anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20074
CF488A Donkey anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20079
CF488A Rabbit anti-chicken IgY (IgG) (H+L), 2 mg/mL    0.5 mL
¥2250 Biotium Inc.
20166
CF488A donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20169
CF488A donkey anti-guinea pig IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20172
CF488A rabbit anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL    0.5mL
¥2700 Biotium Inc.
20204
CF488A monoclonal mouse anti-biotin IgG, 2 mg/mL    0.25mL
¥2700 Biotium Inc.
20208
CF488A chicken anti-mouse IgG(H+L),2 mg/mL    0.5mL
¥2250 Biotium Inc.
20209
CF488A chicken anti-rabbit IgG(H+L),2 mg/mL    0.5mL
¥2250 Biotium Inc.
20210
CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL    0.25mL
¥2925 Biotium Inc.
20215
CF488A monoclonal mouse anti-GFP IgG, 1mg/mL    0.1mL
¥3300 Biotium Inc.
20225
CF488A chicken anti-goat IgG(H+L), 2 mg/mL    0.5mL
¥2250 Biotium Inc.
20228
CF488A Monoclonal Mouse anti-6X His IgG, 1 mg/mL    50uL
¥4275 Biotium Inc.
20238
CF488A Rabbit anti-HA (Hemagglutinin) IgG, 1 mg/mL    50uL
¥4275 Biotium Inc.
20246
CF488A Goat Anti-Mouse IgG1 (1), 2mg/mL    0.25mL
¥2925 Biotium Inc.
20256
CF488A Goat Anti-Mouse IgG2a (2a), 2mg/mL    0.25mL
¥2925 Biotium Inc.
20266
CF488A Goat Anti-Mouse IgG2b (2b), 2mg/mL    0.25mL
¥2925 Biotium Inc.
20421
CF488A Rabbit anti-RFP, 1mg/mL    100uL
¥3300 Biotium Inc.
20424
CF488A Goat anti-GST (Glutathione S-transferase tag), 1mg/mL    100uL
¥3300 Biotium Inc.
20432
CF488A Rabbit anti-FLAG, 1mg/mL    100uL
¥3450 Biotium Inc.
20436
CF488A Rabbit anti-MYC, 1mg/mL    100uL
¥3450 Biotium Inc.
20440
CF488A Rabbit anti-V5, 1mg/mL    100uL
¥3450 Biotium Inc.
92233
Mix-n-Stain CF488A Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
¥1725 Biotium Inc.
92253
Mix-n-Stain CF488A Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
¥1575 Biotium Inc.
92273
Mix-n-Stain CF488A Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
¥1425 Biotium Inc.
20010-1
CF488A goat anti-mouse IgG (H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20010-1mg
CF488A goat anti-mouse IgG (H+L)    1mg
¥2325 Biotium Inc.
20011-1
CF488A F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL    50ul
¥1125 Biotium Inc.
20012-1
CF488A goat anti-rabbit IgG(H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20012-1mg
CF488A goat anti-rabbit IgG(H+L)    1mg
¥2325 Biotium Inc.
20013-1
CF488A F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL    50uL
¥1125 Biotium Inc.
20014-1
CF488A donkey anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20014-1mg
CF488A donkey anti-mouse IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20015-1
CF488A donkey anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20015-1mg
CF488A donkey anti-rabbit IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20016-1
CF488A donkey anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20016-1mg
CF488A donkey anti-goat IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20017-1
CF488A goat anti-guinea pig IgG(H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20017-1mg
CF488A goat anti-guinea pig IgG(H+L)    1mg
¥2325 Biotium Inc.
20018-1
CF488A goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20018-1mg
CF488A goat anti-mouse IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20019-1
CF488A goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20019-1mg
CF488A goat anti-rabbit IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20020-1
CF488A goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20020-1mg
CF488A goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20021-1
CF488A rabbit anti-goat IgG(H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20021-1mg
CF488A rabbit anti-goat IgG(H+L)    1mg
¥2325 Biotium Inc.
20022-1
CF488A goat anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20022-1mg
CF488A goat anti-human IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20023-1
CF488A goat anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20023-1mg
CF488A goat anti-rat IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20024-1
CF488A donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20024-1mg
CF488A donkey anti-sheep IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20025-1
CF488A rabbit anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20025-1mg
CF488A rabbit anti-rat IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20026-1
CF488A rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20026-1mg
CF488A rabbit anti-mouse IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20027-1
CF488A donkey anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20027-1mg
CF488A donkey anti-rat IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20028-1
CF488A goat anti-swine IgG(H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20028-1mg
CF488A goat anti-swine IgG(H+L)    1mg
¥2325 Biotium Inc.
20071-1
CF488A Rabbit anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20071-1mg
CF488A Rabbit anti-human IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20074-1
CF488A Donkey anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20074-1mg
CF488A Donkey anti-human IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20079-1
CF488A Rabbit anti-chicken IgY (IgG) (H+L), 2 mg/mL    50uL
¥900 Biotium Inc.
20079-1mg
CF488A Rabbit anti-chicken IgY (IgG) (H+L)    1mg
¥2325 Biotium Inc.
20166-1
CF488A donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20166-1mg
CF488A donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20169-1
CF488A donkey anti-guinea pig IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20169-1mg
CF488A donkey anti-guinea pig IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20172-1
CF488A rabbit anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20172-1mg
CF488A rabbit anti-sheep IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20204-1
CF488A monoclonal mouse anti-biotin IgG, 2 mg/mL    50uL
¥1275 Biotium Inc.
20208-1
CF488A chicken anti-mouse IgG(H+L),2 mg/mL    50uL
¥900 Biotium Inc.
20208-1mg
CF488A chicken anti-mouse IgG(H+L)    1mg
¥2325 Biotium Inc.
20209-1
CF488A chicken anti-rabbit IgG(H+L),2 mg/mL    50uL
¥900 Biotium Inc.
20209-1mg
CF488A chicken anti-rabbit IgG(H+L)    1mg
¥2325 Biotium Inc.
20210-1
CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL    50uL
¥1425 Biotium Inc.
20225-1
CF488A chicken anti-goat IgG(H+L), 2 mg/mL    50uL
¥975 Biotium Inc.
20225-1mg
CF488A chicken anti-goat IgG(H+L)    1mg
¥2325 Biotium Inc.
20246-1
CF488A Goat Anti-Mouse IgG1 (1), 2mg/mL    50uL
¥1275 Biotium Inc.
20256-1
CF488A Goat Anti-Mouse IgG2a (2a), 2mg/mL    50uL
¥1275 Biotium Inc.
20266-1
CF488A Goat Anti-Mouse IgG2b (2b), 2mg/mL    50uL
¥1275 Biotium Inc.
20293-1
CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL    50uL
¥1125 Biotium Inc.
20293-1mg
CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed    1mg
¥2775 Biotium Inc.
20302-1
CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL    50uL
¥1500 Biotium Inc.
20302-1mg
CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)    1mg
¥3600 Biotium Inc.
20347-250uL
CF488A Goat Anti-Human IgM (mu chain), 2mg/mL    250uL
¥2025 Biotium Inc.
20347-50uL
CF488A Goat Anti-Human IgM (mu chain), 2mg/mL    50uL
¥1125 Biotium Inc.
20428-1mg
CF488A Goat anti-human IgA (alpha chain)    1mg
¥2325 Biotium Inc.
20428-500uL
CF488A Goat anti-human IgA (alpha chain), 2mg/mL    0.5mL
¥2250 Biotium Inc.
20428-50uL
CF488A Goat anti-human IgA (alpha chain), 2mg/mL    50uL
¥900 Biotium Inc.
20444-1mg
CF488A Goat anti-Human IgG (H&L), Fc gamma specific, highly cross adsorbed    1mg
¥2850 Biotium Inc.
20444-500uL
CF488A Goat anti-Human IgG (H&L), Fc gamma specific, highly cross adsorbed    0.5mL
¥2775 Biotium Inc.
20444-50uL
CF488A Goat anti-Human IgG (H&L), Fc gamma specific, highly cross adsorbed    50uL
¥1125 Biotium Inc.
20449-1mg
CF488A Llama anti-rabbit IgG (H&L)    1mg
¥2475 Biotium Inc.
20449-500uL
CF488A Llama anti-rabbit IgG (H&L), 2mg/mL    0.5mL
¥2400 Biotium Inc.
20449-50uL
CF488A Llama anti-rabbit IgG (H&L), 2mg/mL    50uL
¥975 Biotium Inc.
20454-1mg
CF488A Llama anti-mouse IgG (H&L)    1mg
¥2475 Biotium Inc.
20454-500uL
CF488A Llama anti-mouseIgG (H&L), 2mg/mL    0.5mL
¥2400 Biotium Inc.
20454-50uL
CF488A Llama anti-mouseIgG (H&L), 2mg/mL    50uL
¥975 Biotium Inc.


表二:CF488A 染料及其他
00042-T
phalloidin, CF488A    50U
¥975 Biotium Inc.
00042
phalloidin, CF488A    300U
¥4575 Biotium Inc.
00005
alpha-bungarotoxin, CF488A     500uG
¥4500 Biotium Inc.
29005
Annexin V, CF488 conjugate    0.5ML
¥3300 Biotium Inc.
20289
Bovine serum albumin, CF488A conjugate    5x1mg
¥2550 Biotium Inc.
92086
CF488A alkyne    0.5MG
¥3750 Biotium Inc.
92037
CF488A amine    1MG
¥2850 Biotium Inc.
30060
CF488A and 7-AAD Apoptosis Assay Kit    1KIT
¥3000 Biotium Inc.
30061
CF488A and PI Apoptosis Assay Kit    1KIT
¥3000 Biotium Inc.
29060
CF488A Arachis hypogaes Lectin PNA (Peanut)    1mg
¥1875 Biotium Inc.
92080
CF488A azide    0.5MG
¥3750 Biotium Inc.
92075
CF488A BCN    0.5MG
¥4575 Biotium Inc.
92004
CF488A Biocytin    0.5MG
¥4125 Biotium Inc.
92152
CF488A hydrazide    1MG
¥2250 Biotium Inc.
92022
CF488A maleimide    1UMOL
¥3075 Biotium Inc.
92187
CF488A picolyl azide    0.5MG
¥3900 Biotium Inc.
92213
CF488A Protein Labeling Kit "3 labelings"    1KIT
¥5475 Biotium Inc.
30063
CF488A TUNEL Assay Apoptosis Detection Kit    1KIT
¥6000 Biotium Inc.
92171
CF488A Tyramide    0.5MG
¥3750 Biotium Inc.
92051
CF488A, aminooxy    1MG
¥5850 Biotium Inc.
00036
CF488A-cAMP    100ug
¥1425 Biotium Inc.
40067
CF488A-dCTP, lyophilized powder    25nmol
¥4800 Biotium Inc.
80110
CF488A-Dextran 10,000 MW, anionic and fixable    1MG
¥1200 Biotium Inc.
40008
CF488A-dUTP, lyophilized powder    25nmol
¥4800 Biotium Inc.
92177
CF488DI Aminooxy    1MG
¥2700 Biotium Inc.
92166
CF488DI hydrazide    1MG
¥2250 Biotium Inc.
00070
Cholera Toxin Subunit B, CF488A conjugate    100ug
¥2475 Biotium Inc.
29016
Concanavalin A, CF488A conjugate    5x1mg
¥1950 Biotium Inc.
00081
Human Transferrin, CF488A conjugate    1mg
¥1275 Biotium Inc.
92350
Mix-n-Stain CF488A Small Ligand Labeling Kit    1KIT
¥2250 Biotium Inc.
92097
MTS-CF488A    1MG
¥2400 Biotium Inc.
29034
Streptavidin, CF488A conjugate    1mg
¥2400 Biotium Inc.
29022-1
Wheat Germ Agglutinin, CF488A conjugate    1mg
¥900 Biotium Inc.
29022
Wheat Germ Agglutinin, CF488A conjugate    5x1mg
¥3750 Biotium Inc.