Mix-n-Stain CF Dye 抗体快速标记试剂盒

用最亮的荧光染料在30分钟内标记5到100 µg抗体——抗体标记技术的革命!

混合之后即可染色——实验就是如此简单!

    抗体染料,一抗,二抗,山羊抗大鼠IgG,山羊抗鼠IgG,兔抗山羊IgG,兔抗大鼠IgG,兔抗鼠IgG,山羊抗鸡IgG,驴抗山羊IgG,驴抗鼠IgG,驴抗大鼠IgG,驴抗羊IgG,驴抗绵羊IgG,山羊抗兔IgG,羊抗人IgG,蛋白质,偶联抗体

    Mix-n-Stain CF 染料抗体标记试剂盒极大地简化了制备荧光标记抗体,尤其是一抗的标记过程。只需将抗体和CF 染料简单混合于试剂盒内提供的缓冲溶液中即可,整个混合过程可以在30秒内完成。等待30分钟后,无需进一步的分离过程,用户所获得的荧光标记抗体就足以和市场上一些主要品牌的预标记荧光抗体相媲美。而且用户完全不需要计算标记过程需要使用多少染料,只要将抗体和试剂盒内提供的全部染料混合即可,用户总能获得最佳的标记结果。更重要的是,标记反应可以耐受一些主要的抗体稳定试剂,比如:叠氮钠,Tris以及低浓度的甘油,BSA和明胶。

    Mix-n-Stain CF 标记试剂盒的另外一个优势就是Biotium优质的CF 染料家族。大部分CF 染料都比Alexa Fluor®、CY以及其他一些相似的商业化染料更亮,光学稳定性也更好。CF 染料家族的产品众多,光谱波长覆盖了整个可见和近红外区域。因此,在标记商业化的一抗时,用户在选择不同的颜色时有很大的自由,从而使得多波长检测更加容易也更加的经济。

特点:

  • 无需纯化:只需将抗体和优质的CF 染料简单混合于我们提供的缓冲溶液中并等待30分钟即可。标记好的抗体可直接用于染色样品,不需要过滤或者透析。

  • 可耐受NaN3,Tris,甚至BSA和明胶:标记抗体前不需要将抗体溶液中的叠氮钠或Tris移除,亦可以耐受低浓度的BSA和明胶

  • 超过20种CF染料可供选择,从此多荧光检测不再受制于荧光抗体。您的想象有多丰富,仪器的能力有多强,多荧光检测的能力就有多强。


 

图 2. Frozen section of rat testis stained with Mix-n-Stain CF488A labeled mouse anti-tubulin (microtubules, green), Mix-n-Stain CF555 labeled mouse anti-ZO1 (tight junctions, red), and CF640R phalloidin (cat. no. 00050) (actin filaments, cyan).

常见问题和答复:

  • 标记的原理是化学性质的还是物理性质的?
  • 答:标记的原理是化学性质的,也就是说染料是通过共价化学键的方式与抗体之间进行结合。化学共价键的结合方式能够确保在多重染色的情况下,多种被不同CF染料标记试剂盒标记的抗体之间不会发生染料重分布和迁移。

  • 与Invitrogen的Zeon抗体标记试剂盒相比,Mix-n-Stain具有什么优势?
  • 答:优点主要有四个:1)CF染料是通过化学共价键的方式与抗体结合,在多重染色的情况下,能够消除各种染料在不同抗体间的迁移。2)Mix-n-Stain标记的抗体在储存缓冲体系中可以保存几个月的时间,而Zeon只能保持30分钟。3)Mix-n-Stain中的CF染料使标记的抗体偶合物化学构象改变更少,因为CF染料通过化学键直接与抗体偶联,不像Zenon是通过其他抗体片段与目标抗体偶联。4)无需计算染料/蛋白样品比例,只需简单混合和标记即可。

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    订货信息:

    编号 产品名称/规格

    价格(rmb)

    生产商
    92306
    Mix-n-Stain APC Antibody Labeling Kit, 1x(25-50ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92307
    Mix-n-Stain APC Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1875 Biotium Inc.
    92310
    Mix-n-Stain APC-CF750T Antibody Labeling Kit, 1x(25-50ug) labeling    1KIT
    ¥2325 Biotium Inc.
    92311
    Mix-n-Stain APC-CF750T Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥2625 Biotium Inc.
    92244
    Mix-n-Stain Biotin Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92286
    Mix-n-Stain Biotin Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92266
    Mix-n-Stain Biotin Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92250
    Mix-n-Stain CF350 Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92230
    Mix-n-Stain CF350 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92270
    Mix-n-Stain CF350 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92304
    Mix-n-Stain CF405L Antibody Labeling Kit, 1x(20-50ug) labeling    1Kit
    ¥1575 Biotium Inc.
    92305
    Mix-n-Stain CF405L Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92303
    Mix-n-Stain CF405L Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92252
    Mix-n-Stain CF405M Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92232
    Mix-n-Stain CF405M Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92272
    Mix-n-Stain CF405M Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92362
    Mix-n-Stain CF405M Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92251
    Mix-n-Stain CF405S Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92231
    Mix-n-Stain CF405S Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92271
    Mix-n-Stain CF405S Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92356
    Mix-n-Stain CF408 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92233
    Mix-n-Stain CF488A Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92273
    Mix-n-Stain CF488A Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92253
    Mix-n-Stain CF488A Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92350
    Mix-n-Stain CF488A Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92357
    Mix-n-Stain CF500 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92290
    Mix-n-Stain CF532 Antibody Labeling Kit, 1x(20-50ug) labeling    1Kit
    ¥1575 Biotium Inc.
    92291
    Mix-n-Stain CF532 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92289
    Mix-n-Stain CF532 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92358
    Mix-n-Stain CF540 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92267
    Mix-n-Stain CF543 Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92247
    Mix-n-Stain CF543 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92287
    Mix-n-Stain CF543 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92234
    Mix-n-Stain CF555 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92274
    Mix-n-Stain CF555 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92254
    Mix-n-Stain CF555 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92235
    Mix-n-Stain CF568 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92275
    Mix-n-Stain CF568 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92255
    Mix-n-Stain CF568 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92351
    Mix-n-Stain CF568 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92236
    Mix-n-Stain CF594 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92276
    Mix-n-Stain CF594 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92256
    Mix-n-Stain CF594 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92352
    Mix-n-Stain CF594 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92237
    Mix-n-Stain CF633 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92277
    Mix-n-Stain CF633 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92257
    Mix-n-Stain CF633 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92353
    Mix-n-Stain CF633 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92245
    Mix-n-Stain CF640R Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92278
    Mix-n-Stain CF640R Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92258
    Mix-n-Stain CF640R Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92354
    Mix-n-Stain CF640R Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92238
    Mix-n-Stain CF647 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92279
    Mix-n-Stain CF647 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92259
    Mix-n-Stain CF647 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92359
    Mix-n-Stain CF647 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92239
    Mix-n-Stain CF660C Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92280
    Mix-n-Stain CF660C Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92260
    Mix-n-Stain CF660C Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92360
    Mix-n-Stain CF660C Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92243
    Mix-n-Stain CF660R Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92281
    Mix-n-Stain CF660R Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92261
    Mix-n-Stain CF660R Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92282
    Mix-n-Stain CF680 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92240
    Mix-n-Stain CF680 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92262
    Mix-n-Stain CF680 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92361
    Mix-n-Stain CF680 Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92246
    Mix-n-Stain CF680R Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92283
    Mix-n-Stain CF680R Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92263
    Mix-n-Stain CF680R Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92355
    Mix-n-Stain CF680R Small Ligand Labeling Kit    1KIT
    ¥2250 Biotium Inc.
    92241
    Mix-n-Stain CF750 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92284
    Mix-n-Stain CF750 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92264
    Mix-n-Stain CF750 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92242
    Mix-n-Stain CF770 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92285
    Mix-n-Stain CF770 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92265
    Mix-n-Stain CF770 Antibody Labeling Kit,1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92268
    Mix-n-Stain CF790 Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92248
    Mix-n-Stain CF790 Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92288
    Mix-n-Stain CF790 Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92295
    Mix-n-Stain FITC Antibody Labeling Kit, 1x(20-50ug) labeling    1KIT
    ¥1050 Biotium Inc.
    92296
    Mix-n-Stain FITC Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1200 Biotium Inc.
    92294
    Mix-n-Stain FITC Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥900 Biotium Inc.
    92312
    Mix-n-Stain Glucose Oxidase Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT    1KIT
    ¥1725 Biotium Inc.
    92313
    Mix-n-Stain Glucose Oxidase Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT    1KIT
    ¥1875 Biotium Inc.
    92300
    Mix-n-Stain HRP Antibody Labeling Kit, 1x(10-20ug) labeling    1KIT
    ¥1425 Biotium Inc.
    92301
    Mix-n-Stain HRP Antibody Labeling Kit, 1x(25-50ug) labeling    1KIT
    ¥1575 Biotium Inc.
    92302
    Mix-n-Stain HRP Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92308
    Mix-n-Stain PerCP Antibody Labeling Kit, 1x(25-50ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92309
    Mix-n-Stain PerCP Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1875 Biotium Inc.
    92298
    Mix-n-Stain RPE Antibody Labeling Kit, 1x(25-50ug) labeling    1KIT
    ¥1725 Biotium Inc.
    92299
    Mix-n-Stain RPE Antibody Labeling Kit, 1x(50-100ug) labeling    1KIT
    ¥1875 Biotium Inc.
    92297
    Mix-n-Stain RPE Antibody Labeling Kit, 1x(5-20ug) labeling    1KIT
    ¥1575 Biotium Inc.