混合之后即可染色——实验就是如此简单!
Mix-n-Stain CF 染料抗体标记试剂盒极大地简化了制备荧光标记抗体,尤其是一抗的标记过程。只需将抗体和CF 染料简单混合于试剂盒内提供的缓冲溶液中即可,整个混合过程可以在30秒内完成。等待30分钟后,无需进一步的分离过程,用户所获得的荧光标记抗体就足以和市场上一些主要品牌的预标记荧光抗体相媲美。而且用户完全不需要计算标记过程需要使用多少染料,只要将抗体和试剂盒内提供的全部染料混合即可,用户总能获得最佳的标记结果。更重要的是,标记反应可以耐受一些主要的抗体稳定试剂,比如:叠氮钠,Tris以及低浓度的甘油,BSA和明胶。
Mix-n-Stain CF 标记试剂盒的另外一个优势就是Biotium优质的CF 染料家族。大部分CF 染料都比Alexa Fluor®、CY以及其他一些相似的商业化染料更亮,光学稳定性也更好。CF 染料家族的产品众多,光谱波长覆盖了整个可见和近红外区域。因此,在标记商业化的一抗时,用户在选择不同的颜色时有很大的自由,从而使得多波长检测更加容易也更加的经济。
特点:
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图 2. Frozen section of rat testis stained with Mix-n-Stain CF488A labeled mouse anti-tubulin (microtubules, green), Mix-n-Stain CF555 labeled mouse anti-ZO1 (tight junctions, red), and CF640R phalloidin (cat. no. 00050) (actin filaments, cyan). |
常见问题和答复:
答:标记的原理是化学性质的,也就是说染料是通过共价化学键的方式与抗体之间进行结合。化学共价键的结合方式能够确保在多重染色的情况下,多种被不同CF染料标记试剂盒标记的抗体之间不会发生染料重分布和迁移。
答:优点主要有四个:1)CF染料是通过化学共价键的方式与抗体结合,在多重染色的情况下,能够消除各种染料在不同抗体间的迁移。2)Mix-n-Stain标记的抗体在储存缓冲体系中可以保存几个月的时间,而Zeon只能保持30分钟。3)Mix-n-Stain中的CF染料使标记的抗体偶合物化学构象改变更少,因为CF染料通过化学键直接与抗体偶联,不像Zenon是通过其他抗体片段与目标抗体偶联。4)无需计算染料/蛋白样品比例,只需简单混合和标记即可。
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订货信息:
编号 | 产品名称/规格 | 价格(rmb) |
生产商 |
92314 | Mix-n-Stain Alkaline Phosphatase Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1680 | Biotium Inc. |
92315 | Mix-n-Stain Alkaline Phosphatase Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1820 | Biotium Inc. |
92306 | Mix-n-Stain APC Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1610 | Biotium Inc. |
92307 | Mix-n-Stain APC Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1750 | Biotium Inc. |
92310 | Mix-n-Stain APC-CF750T Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥2170 | Biotium Inc. |
92311 | Mix-n-Stain APC-CF750T Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥2450 | Biotium Inc. |
92244 | Mix-n-Stain Biotin Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92286 | Mix-n-Stain Biotin Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92266 | Mix-n-Stain Biotin Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92250 | Mix-n-Stain CF350 Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92230 | Mix-n-Stain CF350 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92270 | Mix-n-Stain CF350 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92304 | Mix-n-Stain CF405L Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1540 | Biotium Inc. |
92305 | Mix-n-Stain CF405L Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92303 | Mix-n-Stain CF405L Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92252 | Mix-n-Stain CF405M Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92232 | Mix-n-Stain CF405M Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92272 | Mix-n-Stain CF405M Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92362 | Mix-n-Stain CF405M Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92251 | Mix-n-Stain CF405S Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92231 | Mix-n-Stain CF405S Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92271 | Mix-n-Stain CF405S Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92356 | Mix-n-Stain CF408 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92317 | Mix-n-Stain CF430 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1834 | Biotium Inc. |
92318 | Mix-n-Stain CF430 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1988 | Biotium Inc. |
92316 | Mix-n-Stain CF430 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92320 | Mix-n-Stain CF440 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1834 | Biotium Inc. |
92321 | Mix-n-Stain CF440 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1988 | Biotium Inc. |
92319 | Mix-n-Stain CF440 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92323 | Mix-n-Stain CF450 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1834 | Biotium Inc. |
92324 | Mix-n-Stain CF450 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1988 | Biotium Inc. |
92322 | Mix-n-Stain CF450 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92233 | Mix-n-Stain CF488A Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92273 | Mix-n-Stain CF488A Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92253 | Mix-n-Stain CF488A Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92350 | Mix-n-Stain CF488A Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92357 | Mix-n-Stain CF500 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92332 | Mix-n-Stain CF514 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1694 | Biotium Inc. |
92333 | Mix-n-Stain CF514 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1834 | Biotium Inc. |
92331 | Mix-n-Stain CF514 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92290 | Mix-n-Stain CF532 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1540 | Biotium Inc. |
92291 | Mix-n-Stain CF532 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92289 | Mix-n-Stain CF532 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92358 | Mix-n-Stain CF540 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92267 | Mix-n-Stain CF543 Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92247 | Mix-n-Stain CF543 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92287 | Mix-n-Stain CF543 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92234 | Mix-n-Stain CF555 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92274 | Mix-n-Stain CF555 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92254 | Mix-n-Stain CF555 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92364 | Mix-n-Stain CF555 Small Ligand Labeling Kit 1KIT |
¥2310 | Biotium Inc. |
92235 | Mix-n-Stain CF568 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92275 | Mix-n-Stain CF568 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92255 | Mix-n-Stain CF568 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92351 | Mix-n-Stain CF568 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92236 | Mix-n-Stain CF594 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92276 | Mix-n-Stain CF594 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92256 | Mix-n-Stain CF594 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92352 | Mix-n-Stain CF594 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92237 | Mix-n-Stain CF633 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92277 | Mix-n-Stain CF633 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92257 | Mix-n-Stain CF633 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92353 | Mix-n-Stain CF633 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92245 | Mix-n-Stain CF640R Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92278 | Mix-n-Stain CF640R Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92258 | Mix-n-Stain CF640R Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92354 | Mix-n-Stain CF640R Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92238 | Mix-n-Stain CF647 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92279 | Mix-n-Stain CF647 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92259 | Mix-n-Stain CF647 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92359 | Mix-n-Stain CF647 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92363 | Mix-n-Stain CF650 Small Ligand Labeling Kit 1KIT |
¥2646 | Biotium Inc. |
92239 | Mix-n-Stain CF660C Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92280 | Mix-n-Stain CF660C Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92260 | Mix-n-Stain CF660C Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92360 | Mix-n-Stain CF660C Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92243 | Mix-n-Stain CF660R Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92281 | Mix-n-Stain CF660R Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92261 | Mix-n-Stain CF660R Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92282 | Mix-n-Stain CF680 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92240 | Mix-n-Stain CF680 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92262 | Mix-n-Stain CF680 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92361 | Mix-n-Stain CF680 Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92246 | Mix-n-Stain CF680R Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92283 | Mix-n-Stain CF680R Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92263 | Mix-n-Stain CF680R Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92355 | Mix-n-Stain CF680R Small Ligand Labeling Kit 1KIT |
¥2212 | Biotium Inc. |
92241 | Mix-n-Stain CF750 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92284 | Mix-n-Stain CF750 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92264 | Mix-n-Stain CF750 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92242 | Mix-n-Stain CF770 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92285 | Mix-n-Stain CF770 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92265 | Mix-n-Stain CF770 Antibody Labeling Kit,1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92268 | Mix-n-Stain CF790 Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92248 | Mix-n-Stain CF790 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92288 | Mix-n-Stain CF790 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1400 | Biotium Inc. |
92329 | Mix-n-Stain Digoxigenin Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1694 | Biotium Inc. |
92330 | Mix-n-Stain Digoxigenin Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1834 | Biotium Inc. |
92328 | Mix-n-Stain Digoxigenin Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92326 | Mix-n-Stain DNP Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1694 | Biotium Inc. |
92327 | Mix-n-Stain DNP Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1834 | Biotium Inc. |
92325 | Mix-n-Stain DNP Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92295 | Mix-n-Stain FITC Antibody Labeling Kit, 1x(20-50ug) labeling 1KIT |
¥1036 | Biotium Inc. |
92296 | Mix-n-Stain FITC Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1176 | Biotium Inc. |
92294 | Mix-n-Stain FITC Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥882 | Biotium Inc. |
92312 | Mix-n-Stain Glucose Oxidase Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1694 | Biotium Inc. |
92313 | Mix-n-Stain Glucose Oxidase Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1750 | Biotium Inc. |
92300 | Mix-n-Stain HRP Antibody Labeling Kit, 1x(10-20ug) labeling 1KIT |
¥1330 | Biotium Inc. |
92301 | Mix-n-Stain HRP Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1470 | Biotium Inc. |
92302 | Mix-n-Stain HRP Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1610 | Biotium Inc. |
92308 | Mix-n-Stain PerCP Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1610 | Biotium Inc. |
92309 | Mix-n-Stain PerCP Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1750 | Biotium Inc. |
92298 | Mix-n-Stain RPE Antibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥1610 | Biotium Inc. |
92299 | Mix-n-Stain RPE Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1750 | Biotium Inc. |
92297 | Mix-n-Stain RPE Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1470 | Biotium Inc. |
92335 | Mix-n-Stain? CF?570 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1694 | Biotium Inc. |
92336 | Mix-n-Stain? CF?570 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1834 | Biotium Inc. |
92334 | Mix-n-Stain? CF?570 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92338 | Mix-n-Stain? CF?583 Antibody Labeling Kit, 1x(20-50ug) labeling 1Kit |
¥1694 | Biotium Inc. |
92339 | Mix-n-Stain? CF?583 Antibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥1834 | Biotium Inc. |
92337 | Mix-n-Stain? CF?583 Antibody Labeling Kit, 1x(5-20ug) labeling 1KIT |
¥1540 | Biotium Inc. |
92400-T | Mix-n-Stain? CF?680T Total Protein Prestain Kit 1KIT |
¥1400 | Biotium Inc. |
92400 | Mix-n-Stain? CF?680T Total Protein Prestain Kit 1KIT |
¥5250 | Biotium Inc. |
92401-T | Mix-n-Stain? CF?770T Total Protein Prestain Kit 1KIT |
¥1400 | Biotium Inc. |
92401 | Mix-n-Stain? CF?770T Total Protein Prestain Kit 1KIT |
¥5250 | Biotium Inc. |
92340 | Mix-n-Stain? RPE-CF?647T Fntibody Labeling Kit, 1x(25-50ug) labeling 1KIT |
¥2310 | Biotium Inc. |
92341 | Mix-n-Stain? RPE-CF?647T Fntibody Labeling Kit, 1x(50-100ug) labeling 1KIT |
¥2590 | Biotium Inc. |